Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.īone marrow basophil progenitors downregulate cell cycle genes during differentiation. An interactive web resource was created to enable the wider research community to explore the expression dynamics for any gene of interest.īasophils differentiation mast cells single-cell RNA sequencing transcriptomics. Cell fate assays showed that multicolor flow cytometry and transcriptional profiling successfully predict the bipotent phenotype of a previously uncharacterized population of peritoneal basophil-mast cell progenitors.Ī combination of molecular and functional profiling of bone marrow and peritoneal cells provided a detailed road map of basophil and mast cell development. Molecular profiling and pseudotime ordering of the single cells revealed gene expression changes during differentiation. We performed multicolor flow cytometry, high-coverage single-cell RNA sequencing analyses, and cell fate assays to chart basophil and mast cell differentiation at single-cell resolution in mouse.Īnalysis of flow cytometry data reconstructed a detailed map of basophil and mast cell differentiation, including a bifurcation of progenitors into two specific trajectories.
Whereas these mature effector cells are extensively studied, the differentiation trajectories from hematopoietic progenitors to basophils and mast cells are largely uncharted at the single-cell level. Basophils and mast cells contribute to the development of allergic reactions.
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